PKM and neoplasm: In in vitro experiments on tumor cells, they have confirmed, using a luciferase reporter gene assay, that TEAD4 boosts the transcription and expression of PKM2, which further elevates the activity of HIF-1α and therefore increases the expression of the HIF-1α-targeted glycolytic genes glucose transporter-1 and hexokinase II; conversely, the supplementation of 2-deoxy-d-glucose (2-DG) blocked the positively regulated glycolysis by TEAD4 and PKM2.59