To verify that USP25 ameliorates ischemic stroke injury by regulating TAB2, recombinant adeno‐associated virus serotype 9 (AAV9) vectors carrying TAB2 siRNA or sequence encoding FLAG‐tagged TAB2 were generated and intracerebroventricularly injected into mice to knockdown or overexpress TAB2 in the brain (Figure8A). Here, USP25 is linked to ischemic stroke.