For example, pro-inflammatory mediators (i.e., TNF, IL1B, IFNG, TLR7), AD-associated genes (i.e., APP, TREM2), metabolic regulators (i.e., MTORC1, IGF1), and proliferation-associated genes (i.e., CSF1, SPI1) were upregulated with age in both sexes and across both isolation methods. The gene discussed is SPI1; the disease is Alzheimer disease.