Since our previous work had found that AKR1B10 was significantly elevated either in PC9-BrM3 cells or in tissue and serum samples of NSCLC patients with BM, we verified the potential association between up-regulated AKR1B10 protein expression and the observed acquired drug resistance of brain metastatic cells. The gene discussed is AKR1B10; the disease is non-small cell lung carcinoma.