CD274 and colorectal carcinoma: Representative IHC images from two patients were shown in Fig. S4A. Since ORC1 contributed the biggest part to the DGRG risk score, and CRC patients with up-regulated ORC1 expression were supposed to demonstrate reduced DGRG risk scores, the present IHC results to some extent confirmed the up-regulated PD-L1 expression and therefore the potentially improved ICI therapeutic sensitivity for low risk patients, which was in consistency with the above bioinformatic analysis results.