To investigate the role of BARX1 in NSCLC, we constructed two stable cell lines expressing shRNA targeting BARX1. The stable knockdown efficiency of the BARX1-shRNA#1 (shBARX1#1) and BARX1-shRNA#2 (shBARX1#2) was verified by Western blotting and RT-qPCR (Fig. 4A, C). The gene discussed is BARX1; the disease is non-small cell lung carcinoma.