We have previously described the evolution of tumor-specific CD8+ T cell response in BALB/c mice 7–22 days after subcutaneous implantation of autologous CT26 colorectal tumors.15 CD8+ T cells of multiple specificities were primed in the tumor-draining lymph nodes (t-DLN), and as the tumor progressed, the ratio of effector to exhausted phenotypes detectable in the t-DLNs decreased. Here, CD8A is linked to colorectal neoplasm.