We performed real-time PCR for Tgfβ1, Tnfα, Colα1, Pdfgrs, Timp-1, CCND2, and Mycn, the key factors for NASH, liver fibrosis, and liver tumor (38) in WT, the dKO, and the tKO mouse livers. This evidence concerns the gene CCND2 and metabolic dysfunction-associated steatohepatitis.