We performed real-time PCR for Tgfβ1, Tnfα, Colα1, Pdfgrs, Timp-1, CCND2, and Mycn, the key factors for NASH, liver fibrosis, and liver tumor (38) in WT, the dKO, and the tKO mouse livers. The gene discussed is TGFB1; the disease is metabolic dysfunction-associated steatohepatitis.