In line with the m6A writer function of METTL3, dot blot demonstrated that METTL3 knockdown decreased RNA m6A in human NAFLD-HCC cell line HKCI2 cells; conversely, ectopic expression of METTL3 increased m6A abundance in NAFLD-HCC cell line HKCI10 cells (Figures 1D and S1E). This evidence concerns the gene METTL3 and hepatocellular carcinoma.