In line with the m6A writer function of METTL3, dot blot demonstrated that METTL3 knockdown decreased RNA m6A in human NAFLD-HCC cell line HKCI2 cells; conversely, ectopic expression of METTL3 increased m6A abundance in NAFLD-HCC cell line HKCI10 cells (Figures 1D and S1E). The gene discussed is METTL3; the disease is metabolic dysfunction-associated steatotic liver disease.