A syngeneic orthotopic NAFLD-HCC model was established by injecting Hepa1-6-Luc cells with or without METTL3 knockout into NASH livers of C57BL/6J mice, followed by treatment with anti-PD-1 or IgG isotype (Figure 7A). Here, METTL3 is linked to metabolic dysfunction-associated steatotic liver disease.