The creation of Phe437Pro and Gln453Arg missense mutations in the DNA-binding domain in medaka myrf [orthologous to human MYRF p.(Phe387Ser) and p.(Gln403His) loci, identified in syndromic patients with CHD (Qi et al., 2018)], using ABE8e, induced moderate proportions of cardiac phenotypes in F0-edited embryos, including abnormal cardiac looping and primarily heart edema, confirming the clinical relevance of missense mutations in the MYRF DNA-binding domain in vivo. This evidence concerns the gene MYRF and coronary artery disorder.