TBX1 and 22q11.2 deletion syndrome: Also, targeting the positive control gene tbx1 yielded a highly penetrant phenotype encompassing looping defect, ventricular malformation and oropharyngeal malformation, in line with principle phenotypic features in zebrafish, and mirroring cardiac and facial DiGeorge syndrome features linked to TBX1 in human (Choudhry and Trede, 2013; Jerome and Papaioannou, 2001; Zhang et al., 2006).