Our phenotypic analysis of 12 preselected genes by CRISPR-Cas9-mediated targeting in medaka highlighted a novel cardiovascular phenotype spectrum of cardiac looping and morphological defects for five CHD-associated genes, mapre2, smg7, cdc42bpab, myrf and ankrd11, including a positive control tbx1 (Table S2). The gene discussed is MAPRE2; the disease is coronary artery disorder.