To sum up, the evaluations by the MTT viability test, colony formation observation, and invasion investigation confirmed each other and provided consistent evidence that the effect of 5 μM CA is similar to that of the 30 μM HIF-1α inhibitor in inhibiting viability, proliferation, and the metastatic capacity of HCT-116 CRC cells. The gene discussed is HIF1A; the disease is colorectal carcinoma.