In addition, we also found that NQO1 is positively correlated with the tumor mutation burden in 7 cancer types and positively related to microsatellite instability in 5 cancer types, indicating NQO1 expression may affect the tumor mutation burden and microsatellite instability levels in different cancers and immuno-therapy responses (Figures 3A, B). This evidence concerns the gene NQO1 and cancer.