To evaluate the activity of iC9.CAR T cells in an AML mouse model, sub-lethally irradiated immunodeficient NOD.Cg-Prkdcscid Il2rgtm1Wjl/SzJ (NSG) mice were transplanted with 0.1 × 106 MOLM-14 cells modified to express high level of CD117 and luciferase. The gene discussed is KIT; the disease is acute myeloid leukemia.