LDHD and esophageal squamous cell carcinoma: To explore the metabolic phenotype of ESCC CSCs driven by CDK7-YAP-LDHD axis, we tested the intracellular glucose using a 100 μM 2-NBDG, which is an analog of glucose labeled with FITC and found that relative glucose uptake efficiency was down-regulated notably after suppressing the expression of CDK7 and YAP in ESCC cells (Supplementary Fig. 7a, b).