RNA sequencing confirmed the overexpression of NeuroD4 mRNA, and western blot analysis confirmed the expression of the Flag-NeuroD4 protein in U251, KNS89, and U87 cells infected with the GFP+NeuroD4 virus at 5 days post infection (dpi) (Supplementary Fig. S1E–G). This evidence concerns the gene NEUROD4 and infection.