According to the ranking and enrichment scores, the top six pathways were shown in Fig. 3E and F. TGFβ signaling pathway, viral myocarditis, Wnt signaling pathway, Hedgehog signaling pathway and allograft rejection were positively correlated with PAH, whereas the tricarboxylic acid (TCA) cycle was negatively correlated with PAH. The gene discussed is TGFB1; the disease is viral myocarditis.