DNASE1L3 and neoplasm: Subsequent quantitative PCR (qPCR) analysis of CD45+ lymphocytes isolated from the dLNs showed that the expression of many upregulated immune genes in intratumoral T cells identified by scRNA-Seq (Supplemental Figure 9D) were significantly reduced or unchanged (but not upregulated) in immune cells from tumor-bearing Dnase1l3-KO mice (Figure 6E and Supplemental Figure 10D).