In addition to a reduction in cellular abundance, GSVA revealed that the top significantly upregulated gene set in this cluster of GZMB+CD8+ T cells from MC38 tumors in Dnase1l3-KO mice was “Primary immunodeficiency” compared with those from WT mice (Supplemental Figure 9C; CD8, cluster 13), suggesting that they are also dysfunctional. This evidence concerns the gene GZMB and inborn error of immunity.