To validate the function of SETD1A in HCC stemness, we established stable HCC cell lines of PLC, Huh-7, and Hep3B with shRNA targeting SETD1A, then confirmed its knockdown by Western blotting and qRT-PCR assay (Supplemental Figure 1, E and F). The gene discussed is SETD1A; the disease is hepatocellular carcinoma.