We used a pooled genome-wide CRISPR/Cas9 knockout library (GeCKO v2) consisting of 58,028 gRNAs targeting 19,009 genes (3 guide RNAs [gRNAs] per gene) to investigate genes contributing to stemness of CD24+CD133+ HCC in the PLC cell line (Figure 1A and Supplemental Figure 1A; supplemental material available online with this article; https://doi.org/10.1172/jci.insight.168375DS1). This evidence concerns the gene PROM1 and hepatocellular carcinoma.