To reveal the molecular mechanism underlying the involvement of hepatic Adgrf1 in NAFLD development, RNA-sequencing analysis was performed on RNA samples extracted from the livers of HFD-fed ASO-NC-treated rAAV-GPF, ASO-NC-treated rAAV-Adgrf1, and ASO-Adgrf1-treated rAAV-Adgrf1 mice. The gene discussed is ADGRF1; the disease is metabolic dysfunction-associated steatotic liver disease.