We quantified the number of IBA1+ microglia, the number of cathepsin B+ cells, the proportion of cathepsin B colocalised with IBA1+ microglia and the relative proportion of IBA+ microglia that contain cathepsin B, in samples of temporal cortex from people who had EOAD, AD-DS or healthy ageing (Fig. 2). Here, AIF1 is linked to Alzheimer disease.