To explore the existence of TLR-TLR crosstalk, we therefore treated wildtype and Tlr4−/− iBMDMs with inhibitors of TLR2 (CU CPT22), TLR3 (TLR3/dsRNA complex inhibitor), and TLR9 (ODN 2088) prior to infection with C. neoformans. Here, TLR4 is linked to infection.