FUT4 and thalassemia: Variant analysis of HBB by NGS revealed 6 variants comprising two exonic variants i.e., a silent variant c.9T>C (p.H3H) observed at CD2 and a non-sense variant c.47G>A (p.W16*) at CD15 which results in the formation of a truncated protein, one 5′ splice site variant c.92+5G>C and three intronic variants c.316-185C>T, c.315+74T>G, c.315+16G>C were detected in thalassemia patients.