Serum alpha-fetoprotein (AFP) > 20 μg/L (P = 0.036, OR = 67.097) and prevalence of hepatitis B virus (HBV) infection (P = 0.020, OR = 153.633) were independent significant factors in predicting DPHCC compared to ICC. The gene discussed is AFP; the disease is intrahepatic cholangiocarcinoma.