Based on the above and the fact that patient outcome is not stratified based on TP53 mutation type (Ahmed et al, 2010), we utilised ID8 ovarian cancer cells knocked out (KO) for Trp53 and Pten, alone or in combination (Fig EV1A; including multiple clones of the double KO, dKO; Walton et al, 2016, 2017). The gene discussed is TP53; the disease is ovarian carcinoma.