In summary, we define the Hh gene expression program using RNA sequencing of (1) NIH3T3 cells treated with Hh ligands (Shh, Dhh, Ihh), Smo-activating oxysterols (24(S),25-EC, 7β,27-DHC, 20-OHC), and SAG (Fig. 1 and Supplementary Table 1–3), (2) 10 matched pairs of tumor and adjacent skin samples from patients with newly diagnosed BCC (Fig. 2, 3b, and Supplementary Table 4), (3) 458 human medulloblastomas (Fig. 4a and Supplementary Table 5), and (4) in vivo genetic and iPSC models of Hh-associated medulloblastoma in mice (Fig. 4b, 5g and Supplementary Table 5, 6). Here, SMO is linked to skin basal cell carcinoma.