Single-cell transcriptomic analyses greatly contribute to our understandings in CRC, including intra-tumor cell heterogeneity (5, 6), cancer cell development driven by oncogenic mitogen-activated protein kinase (7), immunosuppressive landscape fostered by oncogenic mutations (8), immune phenotypic linkage between CRC and liver metastasis (9), and alterations of the TME in CRC with chemotherapy (10) or CSF1R blockade (11). This evidence concerns the gene WNK2 and colorectal carcinoma.