A total of 7,343 cells with 19844 genes passing the quality control stage were obtained for further analysis, and six distinct clusters were identified, corresponding to cancer cells (EPCAM, KRT19, KRT18), T cells (CD3D, CD3E, CD2), B cells (MS4A1, CD79A, MAB1), myeloid cells (CD14, CD68, TYROBP, LYZ), fibroblasts (DCN, COL1A1, THY1), and endothelial cells (RAMP2, CD34, CDH5) (Figures 1D, E, S1A). This evidence concerns the gene COL1A1 and cancer.