A total of 7,343 cells with 19844 genes passing the quality control stage were obtained for further analysis, and six distinct clusters were identified, corresponding to cancer cells (EPCAM, KRT19, KRT18), T cells (CD3D, CD3E, CD2), B cells (MS4A1, CD79A, MAB1), myeloid cells (CD14, CD68, TYROBP, LYZ), fibroblasts (DCN, COL1A1, THY1), and endothelial cells (RAMP2, CD34, CDH5) (Figures 1D, E, S1A). The gene discussed is CD34; the disease is cancer.