For prostate cancer organoid cultivation, A83-01, FGF10, FGF2, prostaglandin E2(PGE2), Nicotinamide and p38 inhibitor SB202190, N-acetylcysteine, B27 and Rho kinase inhibitor Y-27632 were added into the general organoid culture medium. Here, FGF2 is linked to prostate cancer.