To examine whether HOTAIR regulates EMT in liver cancer cells through NUAK1, we transfected cells with the HOTAIR overexpression plasmid (P-HOTAIR) followed by addition of the NUAK1 inhibitor HTH-01-015 and then detected EMT markers E-cadherin, N-cadherin, and Vimentin by western blot. The gene discussed is HOTAIR; the disease is liver cancer.