Subsequent analysis revealed that ALDOB levels in GC tissues with lymph node metastasis (LNM) were decreased than in non-LNM GC tissues (P < 0.001, Figure 1A and 1D), and were inversely associated with the tumor-node-metastasis (TNM) stage of GC patients (P < 0.001, Figure 1E). This evidence concerns the gene ALDOB and metastatic malignant neoplasm in the lymph nodes.