Following the capture of epithelial, escaping, and amoeboid cells, single-cell sequencing and DESeq2 analysis identified the enrichment of many genes previously implicated in tumor escape and metastasis, such as NRP1 (32), BIK (33), and PRKCH (34) as well as some previously unstudied candidates, such as LINC01006 and RP11-273G15.2 (Fig. 4). Here, RNF32-DT is linked to neoplasm.