It has been previously reported that RAS/PI3K/AKT/mTOR pathway plays an important role in tumorigenesis, regulating cell cycle distribution and angiogenesis, which is a potential target in melanoma therapy.36 Therefore, we further explored the regulatory effect in mice melanoma tissue and verified that in vivo α-Mangostin down-regulated the expression of RAS and the phosphorylation of PI3K. This evidence concerns the gene MTOR and melanoma.