We modeled ROC curves for the expression levels of tRF-27-FDXXE6XRK45, CEA, CA199, and CA724 in the sera of 130 GC patients and 115 healthy donors, and analyzed the area under the curve and found that the AUC of tRF-27-FDXXE6XRK45 was 0.805 (95% confidence interval (CI) 0.752-0.859), which was higher than 0.691 (95% CI 0.625-0.758) of CEA, 0.644 (95% CI 0.575-0.713) of CA199 and 0.729 (95% CI 0.667-0.792) of CA724 (Fig. 5A). The gene discussed is CEACAM5; the disease is gastric cancer.