To corroborate the increased gene expression markers associated with inflammation and adipose tissue macrophages in thigh adipose tissue of lipedema patients, immunohistochemistry of CD68+ pan-macrophages, CD86+ M1-like (pro-inflammatory) macrophages (Supplementary Figure S5A), and CD206+ M2-like (anti-inflammatory) macrophages were performed (Figure 4A, Supplementary Figure S5B). This evidence concerns the gene MRC1 and Lipedema.