Since both, complex I inhibition and decreased mGPD activity could lead to NAD+ deficiency, we measured NAD+, NADH and ATP in 22Rv1 and PC3 cells in vivo using high-resolution mass spectrometry (HRMS) and observed decreased levels of NAD+ in PC3 cells (Supplementary Fig. 4B) consistent with an altered NAD+ regeneration and sensitivity leading to malignant phenotypes by promoting clonal cell growth and migration upon loss of STAT3 in triple negative breast cancer [54]. This evidence concerns the gene STAT3 and hyperinsulinemic hypoglycemia, familial, 4.