The effect of tested compounds on the total, referring to the overall abundance of an examined protein and phosphorylated (active) protein levels of kinases from the MAPKs family, including AKT, c-Jun N-terminal kinase (JNK), p38, extracellular signal-regulated kinase 1/2 (ERK1/2), and ribosomal protein S6 kinase (p70S6K), using a bead-based multiplex immunoassay in liver cancer cells lysates was assessed. Here, AKT1 is linked to liver cancer.