Herein, we used hepatic organoids derived from liver biopsies obtained from a Pi*MZ and a Pi*ZZ patient (MZ-ORG and ZZ-ORG) and from a Pi*MM, non-deficient individual (MM-ORG), with the aim of checking whether liver organoid cultures also recapitulate the hepatic steatosis found in patients with AATD and investigating the molecular alterations related to such lipid deposits leading to liver damage. The gene discussed is SERPINA1; the disease is Hepatic steatosis.