NRAS and colorectal carcinoma: Commercial amplicon-based Firefly CRC panel (Accu-Kit CRC01, AccuraGen, Shanghai, China), containing a total of 216 hotspots in KRAS, NRAS, PIK3CA, and BRAF [30], was compared with UltraSEEK Panel (Agena Bioscience, San Diego, CA, USA), containing a total of 97 hotspots in KRAS, NRAS, PIK3CA, BRAF, and EGFR.