At this time (Supplementary Figure S1), a new tumor biopsy coming from a pleural lesion was analyzed for the BRAF V600 mutation both by rtPCR (confirming the negative results obtained from the primary tumor) and by IHC (VE1 antibody specific for the V600E-B-RAF protein) that showed an unexpected positive staining in almost all melanoma cells (Figure 1A). Here, BRAF is linked to neoplasm.