However, it cannot be excluded that the timing and the site of collection might play a role in detecting altered plasma levels of mediators of mast cell degranulation, and it is unknown whether peripheral plasma may reliably reflect cranial release of mast cell mediators and, in turns, mast cell degranulation cannot be completely dismissed as a mediator of PACAP38-induced migraine attacks [39,40]. The gene discussed is ADCYAP1; the disease is migraine disorder.