In this in vitro study, we aimed to clarify whether the different statuses of inflammation affect mature adipocytes (AMCs) and their progenitors, adipose mesenchymal stem cells (ASCs), in a different dose- and time-dependent manner, and if irisin could be recognized as a possible predictive marker of obesity. The gene discussed is FNDC5; the disease is obesity due to melanocortin 4 receptor deficiency.