Immunosignals of tricellulin, MFAP5 and α-catenin were quantified along with signals from the cytoskeletal elements MAP2 and NF-L at the ischemic border zone of the neocortex and subcortex in brain tissues from mice subjected to 24 h of focal cerebral ischemia (Figure 2A). This evidence concerns the gene NEFL and Cerebral ischemia.