The first study showed a significant improvement in total testosterone (p < 0.01), free androgen index (p < 0.01), androstenedione (p < 0.01), sex hormone-binding globulin (p < 0.01), progesterone (p < 0.01), total cholesterol (p = 0.01), low density lipoprotein cholesterol (p < 0.01), triglycerides (p < 0.01), menses frequency (p < 0.01), and waist circumference (p = 0.04) after 6 months of treatment, in which PCOS women received 500 mg berberine twice times daily [31]. This evidence concerns the gene SHBG and polycystic ovary syndrome.