The results showed that in cases of IUGR and preeclampsia, the expression of pregnancy-associated plasma protein-A (PAPPA), soluble fms-like tyrosine kinase-1 (sFLT1), soluble endoglin (sENG), alpha-fetoprotein (AFP), placental growth factor (PGF), and galectin 14 (LGALS14) were already altered in EVT cells obtained before 20 weeks of pregnancy with statistical significance. Here, PAPPA is linked to fetal growth restriction.