To identify biological markers that can be used in minimally invasive approaches to diagnose and monitor glioblastoma, we used a membrane-based affinity binding protocol to isolate EVs from serum samples of 85 glioblastoma IDH1-wt patients and 31 age- and sex-matched control subjects and performed total RNA-seq on EVs. The gene discussed is IDH1; the disease is glioblastoma.