To determine the transcriptional distinction between PTHrP WT and KO tumors from early hyperplasia to late carcinoma stages, we collected mammary tumors from three animals per group (PTHrP WT and KO tumor mice) and normal mammary glands from two animals per group (age-matched PTHrP WT and KO tumor-free) at 6-, 8-, 10-, and 12-week time points, respectively (Figure S2). This evidence concerns the gene PTHLH and neoplasm.