For ER-positive breast cancer, the cutoff points of AGR2 defined by median (cutoff 1), lowest tertiles (cutoff 2), lowest quartiles (cutoff 3) and the optimal cutoff point (cutoff 4) were 270.0, 260.0, 245.0 and 215.0, respectively; the cutoff points of FOXA1 were 285.0, 280.0, 275.0 and 255.0, respectively (Table 2). The gene discussed is FOXA1; the disease is breast cancer.