The equation of the prediction model was Logit (P) = 0.960 × Dukes stage (0 represented Phase C, 1 represented Phase B, and 2 represents Phase A) + 1.283 × Differentiation degree (0 indicated high differentiation, 1 indicated medium differentiation, and 2 indicated low differentiation) + 1.315 × Lymph node metastasis (0 meant yes, and 1 meant no) + 0.942 × CEA (actual value) + 1.170 × Alb (actual value) + 1.882 × D-dimer (actual value) + 0.538 × PLAC1 (0 meant negative, and 1 meant positive) + 1.396 × Netrin-1 (0 meant negative, and 1 meant positive) + 0.959. The gene discussed is PLAC1; the disease is metastatic malignant neoplasm in the lymph nodes.