In addition, anti-MUC16 mAb ch5E6 treatment induced apoptosis in MUC16 expressing PC lines at 48–72 h (SW1990, T3M4) (Supplementary Fig. 2c) and NSCLC lines (SW1573, H2122) lines (Supplementary Fig. 2d) at 24–48 h as evident from 0.8 to 2-fold increase in Annexin V staining intensity (P = 0.0489 in PC; P = 0.00234 in NSCLC) as compared to no effects with huIgG1 treatment. This evidence concerns the gene MUC16 and pachyonychia congenita.