Furthermore, transcriptome analysis showed that ECM homeostasis‐related pathways, including “ECM‐receptor interaction” and “Focal adhesion,” were significantly enrichment in Huh7 cells overexpressing FGF9 and closely replicated the reported transcriptomic hallmarks of NASH‐driven HCC induced by diethylnitrosamine (DEN) treatment and western diet feeding.[28, 36] In addition, in vivo studies confirmed increased ECM‐related gene expression in liver tumors of HFHC diet‐fed FGF9 TG mice. This evidence concerns the gene FGF9 and hepatocellular carcinoma.