To confirm the expression profile of genes obtained with RNA-seq, the transcript levels of four DNA-binding NF-κB components (NFκB1, NFκB2, RELA, and RELB) were measured in mono-cultured and co-cultured JeKo-1 and REC-1 MCL cells using qPCR (Figure 1A). The gene discussed is NFKB1; the disease is mantle cell lymphoma.